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Structure and regulatory role of the C-terminal winged helix domain of the archaeal minichromosome maintenance complex

Wiedemann, Christoph and Szambowska, Anna and Häfner, Sabine and Ohlenschläger, Oliver and Gührs, Karl-Heinz and Görlach, Matthias (2015) Structure and regulatory role of the C-terminal winged helix domain of the archaeal minichromosome maintenance complex. Nucleic acids research, 43 (5). pp. 2958-2967. ISSN 1362-4962

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Official URL: http://nar.oxfordjournals.org/cgi/pmidlookup?view=...

Abstract

The minichromosome maintenance complex (MCM) represents the replicative DNA helicase both in eukaryotes and archaea. Here, we describe the solution structure of the C-terminal domains of the archaeal MCMs of Sulfolobus solfataricus (Sso) and Methanothermobacter thermautotrophicus (Mth). Those domains consist of a structurally conserved truncated winged helix (WH) domain lacking the two typical 'wings' of canonical WH domains. A less conserved N-terminal extension links this WH module to the MCM AAA+ domain forming the ATPase center. In the Sso MCM this linker contains a short α-helical element. Using Sso MCM mutants, including chimeric constructs containing Mth C-terminal domain elements, we show that the ATPase and helicase activity of the Sso MCM is significantly modulated by the short α-helical linker element and by N-terminal residues of the first α-helix of the truncated WH module. Finally, based on our structural and functional data, we present a docking-derived model of the Sso MCM, which implies an allosteric control of the ATPase center by the C-terminal domain.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:1043
Deposited By: PhD Piotr Golec
Deposited On:01 Dec 2015 10:05
Last Modified:01 Dec 2015 10:05

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