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A Functional Role for Aβ in Metal Homeostasis? N-Truncation and High-Affinity Copper Binding

Mital, Mariusz and Wezynfeld, Nina Ewa and Frączyk, Tomasz and Wiloch, Magdalena and Wawrzyniak, Urszula and Bonna, Arkadiusz and Tumpach, Carolin and Barnham, Kevin and Haigh, Cathryn and Bal, Wojciech and Drew, Simon (2015) A Functional Role for Aβ in Metal Homeostasis? N-Truncation and High-Affinity Copper Binding. Angewandte Chemie International Edition, 54 . pp. 10460-10464. ISSN 1521-3773

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/anie.20...


Accumulation of the β-amyloid (Aβ) peptide in extracellular senile plaques rich in copper and zinc is a defining pathological feature of Alzheimer's disease (AD). The Aβ1-x (x=16/28/40/42) peptides have been the primary focus of Cu(II) binding studies for more than 15 years; however, the N-truncated Aβ4-42 peptide is a major Aβ isoform detected in both healthy and diseased brains, and it contains a novel N-terminal FRH sequence. Proteins with His at the third position are known to bind Cu(II) avidly, with conditional log K values at pH 7.4 in the range of 11.0-14.6, which is much higher than that determined for Aβ1-x peptides. By using Aβ4-16 as a model, it was demonstrated that its FRH sequence stoichiometrically binds Cu(II) with a conditional Kd value of 3×10(-14) M at pH 7.4, and that both Aβ4-16 and Aβ4-42 possess negligible redox activity. Combined with the predominance of Aβ4-42 in the brain, our results suggest a physiological role for this isoform in metal homeostasis within the central nervous system.

Item Type:Article
Subjects:Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
R Medicine > RB Pathology
Divisions:Department of Biophysics
ID Code:1078
Deposited By: Tomasz Frączyk
Deposited On:18 Dec 2015 11:47
Last Modified:18 Dec 2015 11:47

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