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CpG underrepresentation and the bacterial CpG-specific DNA methyltransferase M.MpeI

Wojciechowski, Marek and Czapinska, Honorata and Bochtler, Matthias (2013) CpG underrepresentation and the bacterial CpG-specific DNA methyltransferase M.MpeI. Proceedings of the National Academy of Sciences of the United States of America, 110 (1). pp. 105-110. ISSN 1091-6490


Official URL: http://www.pnas.org/cgi/pmidlookup?view=long&pmid=...


Cytosine methylation promotes deamination. In eukaryotes, CpG methylation is thought to account for CpG underrepresentation. Whether scarcity of CpGs in prokaryotic genomes is diagnostic for methylation is not clear. Here, we report that Mycoplasms tend to be CpG depleted and to harbor a family of constitutively expressed or phase variable CpG-specific DNA methyltransferases. The very CpG poor Mycoplasma penetrans and its constitutively active CpG-specific methyltransferase M.MpeI were chosen for further characterization. Genome-wide sequencing of bisulfite-converted DNA indicated that M.MpeI methylated CpG target sites both in vivo and in vitro in a locus-nonselective manner. A crystal structure of M.MpeI with DNA at 2.15-Å resolution showed that the substrate base was flipped and that its place in the DNA stack was taken by a glutamine residue. A phenylalanine residue was intercalated into the "weak" CpG step of the nonsubstrate strand, indicating mechanistic similarities in the recognition of the short CpG target sequence by prokaryotic and eukaryotic DNA methyltransferases.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Bioinformatics
ID Code:1093
Deposited By: Prof. Matthias Bochtler
Deposited On:22 Dec 2015 13:16
Last Modified:21 Oct 2016 09:23

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