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Isolation of monomeric photosystem II that retains the subunit PsbS

Haniewicz, Patrycja and De Sanctis, Daniele and Buechel, Claudia and Schroeder, Wolfgang P. and Loi, Maria Cecilia and Kieselbach, Thomas and Bochtler, Matthias and Piano, Dario (2013) Isolation of monomeric photosystem II that retains the subunit PsbS. Photosynth Res., 118 (3). pp. 199-207.

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Abstract

Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Bioinformatics
ID Code:1094
Deposited By: Prof. Matthias Bochtler
Deposited On:22 Dec 2015 13:11
Last Modified:07 Jan 2016 08:49

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