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Lactic acid bacteria as a surface display platform for Campylobacter jejuni antigens.

Kobierecka, Patrycja and Wyszyńska, Agnieszka and Maruszewska, Marta and Wojtania, Anna and Żylińska, Joanna and Bardowski, Jacek K. and Jagusztyn-Krynicka, Elżbieta Katarzyna (2015) Lactic acid bacteria as a surface display platform for Campylobacter jejuni antigens. Journal of molecular microbiology and biotechnology, 25 (1). pp. 1-10. ISSN 1660-2412

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BACKGROUND Food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. Campylobacter spp. is a very widespread cause of gastroenteritis. Over the past decade there has been increasing interest in the use of lactic acid bacteria (LAB) as mucosal delivery vehicles. They represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens. METHODS We examined the binding ability of hybrid proteins to nontreated or trichloroacetic acid (TCA)-pretreated LAB cells by immunofluorescence and Western blot analysis. RESULTS In this study we evaluated the possibility of using GEM (Gram-positive enhancer matrix) particles of Lactobacillus salivarius as a binding platform for 2 conserved, immunodominant, extracytoplasmic Campylobacter jejuni proteins: CjaA and CjaD. We analyzed the binding ability of recombinant proteins that contain C. jejuni antigens (CjaA or CjaD) fused with the protein anchor (PA) of the L. lactis peptidoglycan hydrolase AcmA, which comprises 3 LysM motifs and determines noncovalent binding to the cell wall peptidoglycan. Both fused proteins, i.e. 6HisxCjaAx3LysM and 6HisxCjaDx3LysM, were able to bind to nontreated or TCA-pretreated L. salivarius cells. CONCLUSION Our results documented that the LysM-mediated binding system allows us to construct GEM particles that present 2 C. jejuni antigens.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:1121
Deposited By: dr Tamara Aleksandrzak-Piekarczyk
Deposited On:12 Jan 2016 11:37
Last Modified:12 Jan 2016 11:37

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