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Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids

Moskot, Marta and Jakóbkiewicz-Banecka, Joanna and Kloska, Anna and Smolińska, Elwira and Mozolewski, Paweł and Malinowska, Marcelina and Rychłowski, Michał and Banecki, Bogdan and Węgrzyn, Grzegorz and Gabig-Cimińska, Magdalena (2015) Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids. Scientific Reports, 5 . p. 9378.


Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC436972...


Flavonoids were found previously to modulate efficiency of synthesis of glycosaminoglycans (GAGs), compounds which are accumulated in cells of patients suffering from mucopolysaccharidoses (MPSs). The aim of this work was to determine effects of different flavonoids (genistein, kaempferol, daidzein) used alone or in combinations, on expression of genes coding for proteins involved in GAG metabolism. Analyses with DNA microarray, followed by real-time qRT-PCR revealed that genistein, kaempferol and combination of these two compounds induced dose- and time-dependent remarkable alterations in transcript profiles of GAG metabolism genes in cultures of wild-type human dermal fibroblasts (HDFa). Interestingly, effects of the mixture of genistein and kaempferol were stronger than those revealed by any of these compounds used alone. Similarly, the most effective reduction in levels of GAG production, in both HDFa and MPS II cells, was observed in the presence of genistein, keampferol and combination of these compounds. Forty five genes were chosen for further verification not only in HDFa, but also inMPS II fibroblasts by using real-time qRT-PCR. Despite effects on GAG metabolism-related genes, we found that genistein, kaempferol and mixture of these compounds significantly stimulated expression of TFEB. Additionally, a decrease inMTOR transcript level was observed at these conditions.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:1137
Deposited By: Prof. Magdalena Gabig-Cimińska
Deposited On:02 Mar 2016 10:28
Last Modified:02 Mar 2016 10:28

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