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Identification of miniature plasmids in psychrophilic Arctic bacteria of the genus Variovorax

Ciok, Anna and Dziewit, Lukasz and Grzesiak, Jakub and Budzik, Karol and Górniak, Dorota and Zdanowski, Marek K and Bartosik, Dariusz (2016) Identification of miniature plasmids in psychrophilic Arctic bacteria of the genus Variovorax. FEMS Microbiology Ecology, 92 (4). ISSN 1574-6941

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Official URL: https://femsec.oxfordjournals.org/content/92/4/fiw...


The Svalbard archipelago (Spitsbergen Island) is the northernmost landmass in the European Arctic and has a variety of small- and medium-sized glaciers. The plasmidome of eleven psychrophilic strains of Variovorax spp. isolated from the ice surface of Hans and Werenskiold Glaciers of Spitsbergen Island, was defined. This analysis revealed the presence of six plasmids whose nucleotide sequences have been determined. Four of them, exhibiting high reciprocal sequence similarity, possess unique structures, since their genomes lack any recognized genes. These miniature replicons, not exceeding 1 kb in size, include pHW69V1 (746 bp), which is the smallest autonomous replicon so far identified in free-living bacteria. The miniature plasmids share no similarity with known sequences present in the databases. In silico and experimental analyses identified conserved DNA regions essential for the initiation of replication of these replicons.

Item Type:Article
Uncontrolled Keywords:miniature plasmid, Variovorax, psychrophile, Arctic, glacier, replication system
Subjects:Q Science > QR Microbiology
Divisions:Department of Antarctic Biology
ID Code:1180
Deposited By: prof. Marek K Zdanowski
Deposited On:27 Apr 2016 08:22
Last Modified:08 Mar 2018 15:33

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