![Fig. 1.](F1.medium.gif)
CotH proteins share sequence similarity with known protein kinases. (A) Phylogenetic tree depicting the eukaryotic secretory pathway kinases and related prokaryotic proteins: Fam20A/B, Fam198A/B, four-jointed box 1 (Fjx1), and HipA. Sequence logos were created by WebLogo, using MUSCLE multiple sequence alignment of 165 CotH homologs depicting the loop between strands β1 and β2 (B, Gly-rich loop) and the active site residues (C).