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A meiosis-specific Spt5 homolog involved in non-coding transcription

Gruchota, Julita and Denby Wilkes, Cyril and Arnaiz, Olivier and Sperling, Linda and Nowak, Jacek K. (2017) A meiosis-specific Spt5 homolog involved in non-coding transcription. Nucleic acids research . ISSN 1362-4962 (In Press)

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Official URL: http://nar.oxfordjournals.org/content/early/2017/0...


Spt5 is a conserved and essential transcriptional regulator that binds directly to RNA polymerase and is involved in transcription elongation, polymerase pausing and various co-transcriptional processes. To investigate the role of Spt5 in non-coding transcription, we used the unicellular model Paramecium tetraurelia. In this ciliate, development is controlled by epigenetic mechanisms that use different classes of non-coding RNAs to target DNA elimination. We identified two SPT5 genes. One (STP5v) is involved in vegetative growth, while the other (SPT5m) is essential for sexual reproduction. We focused our study on SPT5m, expressed at meiosis and associated with germline nuclei during sexual processes. Upon Spt5m depletion, we observed absence of scnRNAs, piRNA-like 25 nt small RNAs produced at meiosis. The scnRNAs are a temporal copy of the germline genome and play a key role in programming DNA elimination. Moreover, Spt5m depletion abolishes elimination of all germline-limited sequences, including sequences whose excision was previously shown to be scnRNA-independent. This suggests that in addition to scnRNA production, Spt5 is involved in setting some as yet uncharacterized epigenetic information at meiosis. Our study establishes that Spt5m is crucial for developmental genome rearrangements and necessary for scnRNA production.

Item Type:Article
Uncontrolled Keywords:non-coding RNA, DNA rearangements, transcription regulator, sRNA, ciliates
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Laboratory of DNA Sequencing and Oligonucleotide Synthesis
ID Code:1260
Deposited By: PhD Jacek Nowak
Deposited On:05 Jan 2017 10:38
Last Modified:05 Jan 2017 10:38

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