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An Interplay of S-Nitrosylation and Metal Ion Binding for Astrocytic S100B Protein

Bajor, Małgorzata and Zaręba-Kozioł, Monika and Zhukova, Liliya and Goryca, Krzysztof and Poznański, Jarosław and Wysłouch-Cieszyńska, Aleksandra (2016) An Interplay of S-Nitrosylation and Metal Ion Binding for Astrocytic S100B Protein. PLOS ONE, 11 (5). e0154822. ISSN 1932-6203

Available under License Creative Commons Public Domain Dedication.


Official URL: http://doi.org/10.1371/journal.pone.0154822


Mammalian S100B protein plays multiple important roles in cellular brain processes. The protein is a clinically used marker for several pathologies including brain injury, neurodegeneration and cancer. High levels of S100B released by astrocytes in Down syndrome patients are responsible for reduced neurogenesis of neural progenitor cells and induction of cell death in neurons. Despite increasing understanding of S100B biology, there are still many questions concerning the detailed molecular mechanisms that determine specific activities of S100B. Elevated overexpression of S100B protein is often synchronized with increased nitric oxide-related activity. In this work we show S100B is a target of exogenous S-nitrosylation in rat brain protein lysate and identify endogenous S-nitrosylation of S100B in a cellular model of astrocytes. Biochemical studies are presented indicating S-nitrosylation tunes the conformation of S100B and modulates its Ca2+ and Zn2+ binding properties. Our in vitro results suggest that the possibility of endogenous S-nitrosylation should be taken into account in the further studies of in vivo S100B protein activity, especially under conditions of increased NO-related activity.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Biophysics
ID Code:1264
Deposited By: Prof Jaroslaw Poznanski
Deposited On:23 Dec 2016 09:16
Last Modified:23 Dec 2016 09:16

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