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The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.

Tomecki, Rafał and Kristiansen, Maiken S. and Lykke-Andersen, Søren and Chlebowski, Aleksander and Larsen, Katja M. and Szczęsny, Roman J. and Drążkowska, Karolina and Pastuła, Agnieszka and Andersen, Jens S. and Stępień, Piotr P. and Dziembowski, Andrzej and Jensen, Torben H. (2010) The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L. EMBO Journal, 29 (14). pp. 2342-2357.

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Official URL: http://www.nature.com/emboj/journal/v29/n14/pdf/em...


The eukaryotic RNA exosome is a ribonucleolytic complex involved in RNA processing and turnover. It consists of a nine-subunit catalytically inert core that serves a structural function and participates in substrate recognition. Best defined in Saccharomyces cerevisiae, enzymatic activity comes from the associated subunits Dis3p (Rrp44p) and Rrp6p. The former is a nuclear and cytoplasmic RNase II/R-like enzyme, which possesses both processive exo- and endonuclease activities, whereas the latter is a distributive RNase D-like nuclear exonuclease. Although the exosome core is highly conserved, identity and arrangements of its catalytic subunits in different vertebrates remain elusive. Here, we demonstrate the association of two different Dis3p homologs--hDIS3 and hDIS3L--with the human exosome core. Interestingly, these factors display markedly different intracellular localizations: hDIS3 is mainly nuclear, whereas hDIS3L is strictly cytoplasmic. This compartmental distribution reflects the substrate preferences of the complex in vivo. Both hDIS3 and hDIS3L are active exonucleases; however, only hDIS3 has retained endonucleolytic activity. Our data suggest that three different ribonucleases can serve as catalytic subunits for the exosome in human cells.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Biophysics
ID Code:128
Deposited By: Rafal Tomecki
Deposited On:05 May 2011 20:38
Last Modified:28 Mar 2012 20:35

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