IBB PAS Repository

Arabidopsis SWI/SNF chromatin remodeling complex binds both promoters and terminators to regulate gene expression

Archacki, Rafal and Yatusevich, Ruslan and Buszewicz, Daniel and Krzyczmonik, Katarzyna and Patryn, Jacek and Iwanicka-Nowicka, Roksana and Biecek, Przemysław and Wilczynski, Bartek and Koblowska, Marta and Jerzmanowski, Andrzej and Świeżewski, Szymon (2016) Arabidopsis SWI/SNF chromatin remodeling complex binds both promoters and terminators to regulate gene expression. Nucleic Acids Research .


Official URL: http://nar.oxfordjournals.org/content/early/2016/1...


ATP-dependent chromatin remodeling complexes are important regulators of gene expression in Eukaryotes. In plants, SWI/SNF-type complexes have been shown critical for transcriptional control of key developmental processes, growth and stress responses. To gain insight into mechanisms underlying these roles, we performed whole genome mapping of the SWI/SNF catalytic subunit BRM in Arabidopsis thaliana, combined with transcript profiling experiments. Our data showthatBRM occupies thousands of sites in Arabidopsis genome, most of which located within or close to genes. Among identified direct BRM transcriptional targets almost equal numbers were up- and downregulated upon BRM depletion, suggesting that BRM can act as both activator and repressor of gene expression. Interestingly, in addition to genes showing canonical pattern of BRM enrichment near transcription start site, many other genes showed a transcription termination sitecentred BRM occupancy profile. We found that BRMbound 3� gene regions have promoter-like features, including presence of TATA boxes and high H3K4me3 levels, and possess high antisense transcriptional activity which is subjected to both activation and repression by SWI/SNF complex. Our data suggest that binding to gene terminators and controlling transcription of non-coding RNAs is another way through which SWI/SNF complex regulates expression of its targets.

Item Type:Article
Subjects:Q Science > QH Natural history > QH426 Genetics
Divisions:Department of Protein Biosynthesis
ID Code:1299
Deposited By: dr Roksana Iwanicka-Nowicka
Deposited On:17 Jan 2017 13:48
Last Modified:17 Jan 2017 13:48

Repository Staff Only: item control page