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Myogenic Differentiation of Mouse Embryonic Stem Cells That Lack a Functional Pax7 Gene

Czerwinska, Areta M. and Grabowska, Iwona and Archacka, Karolina and Bem, Joanna and Swierczek, Barbara and Helinska, Anita and Stremińska, Władysława and Fogtman, Anna and Iwanicka-Nowicka, Roksana and Koblowska, Marta and Ciemerych, Maria A. (2016) Myogenic Differentiation of Mouse Embryonic Stem Cells That Lack a Functional Pax7 Gene. STEM CELLS AND DEVELOPMENT .

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The transcription factor Pax7 plays a key role during embryonic myogenesis and sustains the proper function of satellite cells, which serve as adult skeletal muscle stem cells. Overexpression of Pax7 has been shown to promote the myogenic differentiation of pluripotent stem cells. However, the effects of the absence of functional Pax7 in differentiating embryonic stem cells (ESCs) have not yet been directly tested. Herein, we studied mouse stem cells that lacked a functional Pax7 gene and characterized the differentiation of these stem cells under conditions that promoted the derivation of myoblasts in vitro. We analyzed the expression of myogenic factors, such as myogenic regulatory factors and muscle-specific microRNAs, in wild-type and mutant cells. Finally, we compared the transcriptome of both types of cells and did not find substantial differences in the expression of genes related to the regulation of myogenesis. As a result, we showed that the absence of functional Pax7 does not prevent the in vitro myogenic differentiation of ESCs.

Item Type:Article
Subjects:Q Science > QH Natural history > QH426 Genetics
Divisions:Department of Protein Biosynthesis
ID Code:1304
Deposited By: dr Roksana Iwanicka-Nowicka
Deposited On:17 Jan 2017 14:14
Last Modified:01 Mar 2018 11:43

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