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RNA channelling by the eukaryotic exosome

Malet, Hélène and Topf, Maya and Clare, Daniel K. and Ebert, Judith and Bonneau, Fabien and Basquin, Jerome and Drążkowska, Karolina and Tomecki, Rafał and Dziembowski, Andrzej and Conti, Elena and Saibil, Helen R. and Lorenzten, Esben (2010) RNA channelling by the eukaryotic exosome. EMBO Reports, 11 (12). pp. 936-942.


Official URL: http://www.nature.com/embor/journal/v11/n12/pdf/em...


The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Biophysics
ID Code:131
Deposited By: Rafal Tomecki
Deposited On:05 May 2011 20:39
Last Modified:28 Mar 2012 20:35

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