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Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of L-Phenylalanine

Mierzejewska, Jolanta and Tymoszewska, Aleksandra and Chreptowicz, Karolina and Krol, Kamil (2017) Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of L-Phenylalanine. Journal of Molecular Microbiology and Biotechnology, 27 (2). pp. 81-90. ISSN 1660-2412

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Official URL: https://www.karger.com/Journal/Home/228391

Abstract

2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratory Saccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid, S. cerevisiae AM1-d, was selected. Within the 72-h batch culture in a medium containing 5 g/L of L-phenylalanine, AM1-d produced 3.83 g/L of 2-PE in a shaking flask. In this way, we managed to select the diploid S. cerevisiae AM1-d strain, showing a 3- and 5-fold increase in 2-PE production in comparison to parental strains. Remarkably, the enhanced production of 2-PE by the hybrid of 2 yeast laboratory strains is demonstrated here for the first time.

Item Type:Article
Uncontrolled Keywords:2-Phenylethanol; Biotechnology; Biotransformation; Diploid; Yeast
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Laboratory of Mutagenesis and DNA Repair
ID Code:1335
Deposited By: Ph.D Kamil Król
Deposited On:26 May 2017 07:01
Last Modified:08 Mar 2018 15:34

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