Display Settings:

Format

Send to:

Choose Destination
  • Your browser version may not work well with NCBI's Web applications. More information here...

Structure. 2010 Sep 8;18(9):1075-82.

Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.

Source

IBBMC-CNRS UMR8619, IFR 115, Bât. 430, Université Paris-Sud, 91405 Orsay, France.

Abstract

For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection of primary data, or by literature searches. These complexes were validated with two straightforward and efficient biochemical assays, and heterologous expression technologies of complex components were then used to produce the complexes to assess their feasibility experimentally. Approximately one-half of the selected complexes were useful for structural studies, and we detail one particular success story. Our results underscore the importance of accurate target selection and validation in avoiding transient, unstable, or simply nonexistent complexes from the outset.

Copyright © 2010 Elsevier Ltd. All rights reserved.

PMID:
20826334
[PubMed - indexed for MEDLINE]

Supplemental Content

Click here to read

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Write to the Help Desk