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Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis1

Jóźwiak, Adam and Lipko, Agata and Kania, Magdalena and Danikiewicz, Witold and Surmacz, Liliana and Witek, Agnieszka and Wójcik, Jacek and Zdanowski, Konrad and Pączkowski, Cezary and Chojnacki, Tadeusz and Poznański, Jarosław and Swiezewska, Ewa (2017) Modeling of Dolichol Mass Spectra Isotopic Envelopes as a Tool to Monitor Isoprenoid Biosynthesis1. Plant Physiology, 174 . pp. 857-874. ISSN 0032-0889

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Official URL: http://www.plantphysiol.org/content/early/2017/04/...


The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel in plants to generate isoprenoid precursors, has been studied extensively. Elucidation of the isoprenoid metabolic pathways is indispensable for the rational design of plant and microbial systems for the production of industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling of mass spectra of metabolically labeled dolichols (Dols), designed to quantitatively follow the cooperation of MVA and MEP reprogrammed upon osmotic stress (sorbitol treatment) in Arabidopsis (Arabidopsis thaliana). The contribution of the MEP pathway increased significantly (reaching 100%) exclusively for the dominating Dols, while for long-chain Dols, the relative input of the MEP and MVA pathways remained unchanged, suggesting divergent sites of synthesis for dominating and long-chain Dols. The analysis of numerically modeled Dol mass spectra is a novel method to follow modulation of the concomitant activity of isoprenoid-generating pathways in plant cells; additionally, it suggests an exchange of isoprenoid intermediates between plastids and peroxisomes.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Lipid Biochemistry
ID Code:1341
Deposited By: Ewa Swiezewska
Deposited On:09 Jun 2017 09:01
Last Modified:09 Jun 2017 09:01

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