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Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus

Stachyra, Anna and Rak, Monika and Redkiewicz, Patrycja and Madeja, Zbigniew and Gawarecka, Katarzyna and Chojnacki, Tadeusz and Swiezewska, Ewa and Masnyk, Marek and Chmielewski, Marek and Sirko, Agnieszka and Góra-Sochacka, Anna (2017) Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus. Virology Journal, 14 (1). ISSN 1743-422X

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Official URL: http://doi.org/10.1186/s12985-017-0838-x


Background: Cationic derivatives of polyprenols (trimethylpolyprenylammonium iodides – PTAI) with variable chain length between 6 and 15 isoprene units prepared from naturally occurring poly-cis-prenols were tested as DNA vaccine carriers in chickens and mice. This study aimed to investigate if PTAI could be used as an efficient carrier of a DNA vaccine. Methods: Several vaccine mixtures were prepared by combining different proportions of the vaccine plasmid (carrying cDNA encoding a vaccine antigen, hemagglutinin from H5N1 influenza virus) and various compositions of PTAI. The vaccines were delivered by intramuscular injection to either chickens or mice. The presence of specific antibodies in sera collected from the immunized animals was analyzed by enzyme-linked immunosorbent assay (ELISA) and hemagglutination inhibition (HI) test. Results: The mixtures of PTAI with helper lipids, such as DOPE (1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine), DC-cholesterol [{3ß-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol} hydrochloride] or DOPC (1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) induced strong humoral response to the antigen encoded by the DNA vaccine plasmid. Conclusion: The animal immunization results confirmed that PTAI compositions, especially mixtures of PTAI with DOPE and DC-cholesterol, do work as effective carriers of DNA vaccines, comparable to the commercially available lipid transfection reagent.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
ID Code:1377
Deposited By: dr Anna Gora-Sochacka
Deposited On:13 Sep 2017 07:07
Last Modified:13 Sep 2017 07:07

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