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Evidence for interactions between homocysteine and genistein: insights into stroke risk and potential treatment

Banecka-Majkutewicz, Zyta and Kadziński, Leszek and Grabowski, Michał and Bloch, Sylwia and Kaźmierkiewicz, Rajmund and Jakóbkiewicz-Banecka, Joanna and Gabig-Cimińska, Magdalena and Węgrzyn, Grzegorz and Węgrzyn, Alicja and Banecki, Bogdan (2017) Evidence for interactions between homocysteine and genistein: insights into stroke risk and potential treatment. Metabolic Brain Disease, 32 (6). pp. 1855-1860.


Official URL: https://link.springer.com/article/10.1007%2Fs11011...


Elevated plasma homocysteine (2-amino-4- sulfanylbutanoic acid) level is a risk factor for stroke. Moreover, it has been suggested that high levels of homocysteine in the acute phase of an ischemic stroke can predict mortality, especially in stroke patients with the large-vessel atherosclerosis subtype. In clinical studies, supplementation with genistein(5, 7-dihydroxy-3- (4-hydroxyphenyl)-4H-1-benzopyran-4-one) decreased plasma homocysteine levels considerably. Therefore, genistein could be considered as a potential drug for prevention and/or treatment of stroke. However, the mechanism of the effect of genistein on homocysteine level remains to be elucidated. In this report, direct functional interactions between homocysteine and genistein are demonstrated in in vitro experimental systems for determination of methylenetetrahydrofolate reductase (MetF) and glutathione peroxidase(GPx) activities, reconstructed with purified compounds, and in a simple in vivo system, based on measurement of growth rate of Vibrio harveyi and Bacillus subtilis cultures. Results of molecular modelling indicated that homocysteine can directly interact with genistein. Therefore, genisteinmediated decrease in plasma levels of homocysteine, and alleviation of biochemical and physiological effects of one of these compounds by another, might be ascribed to formation of homocysteine-genistein complexes in which biological activities of these molecules are abolished or alleviated.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:1473
Deposited By: Prof. Magdalena Gabig-Cimińska
Deposited On:15 Jan 2018 09:35
Last Modified:27 Jul 2018 22:05

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