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Detection of Plant miRNAs Abundance in Human Breast Milk

Lukasik, Anna and Brzozowska, Iwona and Zielenkiewicz, Urszula and Zielenkiewicz, Piotr (2018) Detection of Plant miRNAs Abundance in Human Breast Milk. International Journal of Molecular Sciences, 19 (1). p. 37. ISSN 1422-0067

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Official URL: http://www.mdpi.com/1422-0067/19/1/37/htm


Breast milk is a natural food and important component of infant nutrition. Apart from the alimentary substances, breast milk contains many important bioactive compounds, including endogenous microRNA molecules (miRNAs). These regulatory molecules were identified in various mammalian biological fluids and were shown to be mostly packed in exosomes. Recently, it was revealed that plant food-derived miRNAs are stably present in human blood and regulate the expression of specific human genes. Since then, the scientific community has focused its efforts on contradicting or confirming this discovery. With the same intention, qRT-PCR experiments were performed to evaluate the presence of five plant food-derived miRNAs (miR166a, miR156a, miR157a, miR172a and miR168a) in breast milk (whole milk and exosomes) from healthy volunteers. In whole milk samples, all examined miRNAs were identified, while only two of these miRNAs were confirmed to be present in exosomes. The plant miRNA concentration in the samples ranged from 4 to 700 fM. Complementary bioinformatics analysis suggests that the evaluated plant miRNAs may potentially influence several crucial biological pathways in the infant organism.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Bioinformatics
ID Code:1486
Deposited By: Anna Łukasik
Deposited On:19 Jan 2018 14:02
Last Modified:19 Jan 2018 14:02

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