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Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition.

Kruszewska, Joanna S. and Gorka-Niec, Wioletta and Kania, Anna and Perlinska-Lenart, Urszula and Smolenska-Sym, Gabriela and Palamarczyk, Grazyna (2011) Integration of additional copies of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition. Fungal Biology, 115 . pp. 124-132.

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

In fungi, transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases. Integration of additional copies of the pmt1 gene into Trichoderma reesei genome unexpectedly resulted in the silencing of pmt1 expression. Strains carrying the additional copies of pmt1 gene exhibited lower total activity of protein O-mannosyltransferases, lower O- and N-glycosylation of secreted proteins and showed defects in their cell wall composition. Moreover, the strains grew slowly on solid medium and were hypersensitive to an antifungal reagent, Calcofluor white. These results indicate that protein O-manosyltransferases are required for proper cell wall formation, and their decreased activity influences not only O- but also N-glycosylation.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Laboratory of Fungal Glycobiology
ID Code:149
Deposited By: prof dr hab Joanna S. Kruszewska
Deposited On:14 Jul 2011 07:17
Last Modified:17 Oct 2014 12:52

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