IBB PAS Repository

Effect of Medicago sativa Mhb1gene expression on defense response of Arabidopsis thaliana plants

Maassen, Anna and Hennig, Jacek (2011) Effect of Medicago sativa Mhb1gene expression on defense response of Arabidopsis thaliana plants. Acta biochimica Polonica, 58 (3). ISSN 1734-154X (In Press)

PDF - Accepted Version


Besides the previously described nitric oxide-detoxification activity we identified new features of class-1 non-symbiotic hemoglobin from Medicago sativa (Mhb1). Under in vitro conditions, using peroxidase in-gel activity assay, the Mhb1 protein was shown to possess also peroxidase-like activity. Due to this activity, in the presence of nitrite and hydrogen peroxide, the protein can mediate autonitration and nitration of other proteins at tyrosine residues, as revealed by tandem mass spectrometry and immune assay approaches. Mhb1 through its multifunctional activities can affect different components of signal transduction cascades operating during plant response to infections. This influence is manifested by Mhb1-mediated selective up-regulation of expression of certain pathogen inducible genes in Pseudomonas syringae infected Arabidopsis thaliana plants which overproduce Mhb1, as revealed by reverse transcription–quantitative real-time PCR analysis. Changes in expression level of these genes can influence such processes as synthesis of secondary metabolites, protein degradation and biosynthesis of ethylene. They can also result in alteration of pathogen-induced defense response of Mhb1 transgenic plants.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Divisions:Laboratory of Plant Pathogenesis
ID Code:157
Deposited By: Prof Jacek Hennig
Deposited On:21 Aug 2011 18:32
Last Modified:28 Mar 2012 20:36

Repository Staff Only: item control page