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Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley.

Sobieszczuk-Nowicka, Ewa and Wrzesiński, Tomasz and Bagniewska-Zadworna, Agnieszka and Kubala, Szymon and Rucińska-Sobkowiak, Renata and Polcyn, Władysław and Misztal, Lucyna and Mattoo, Autar K. (2018) Physio-Genetic Dissection of Dark-Induced Leaf Senescence and Timing Its Reversal in Barley. Plant Physiology, 178 (2). pp. 654-671. ISSN 0032-0889

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Official URL: http://doi.org/10.1104/pp.18.00516


Barley crop model was analyzed for early and late events during the dark-induced leaf senescence (DILS) as well as for deciphering critical time limit for reversal of the senescence process. Chlorophyll fluorescence vitality index Rfd was determined as the earliest parameter that correlated well with the cessation of photosynthesis prior to microautophagy symptoms, initiation of DNA degradation, and severalfold increase in the endonuclease BNUC1. DILS was found characterized by up-regulation of processes that enable recycling of degraded macromolecules and metabolites, including increased NH4+ remobilization, gluconeogenesis, glycolysis, and partial up-regulation of glyoxylate and tricarboxylate acid cycles. The most evident differences in gene medleys between DILS and developmental senescence included hormone-activated signaling pathways, lipid catabolic processes, carbohydrate metabolic processes, low-affinity ammonia remobilization, and RNA methylation. The mega-autophagy symptoms were apparent much later, specifically on day 10 of DILS, when disruption of organelles—nucleus and mitochondria —became evident. Also, during this latter-stage programmed cell death processes, namely, shrinking of the protoplast, tonoplast interruption, and vacuole breakdown, chromatin condensation, more DNA fragmentation, and disintegration of the cell membrane were prominent. Reversal of DILS by re-exposure of the plants from dark to light was possible until but not later than day 7 of dark exposure and was accompanied by regained photosynthesis, increase in chlorophyll, and reversal of Rfd, despite activation of macro-autophagy-related genes.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
ID Code:1575
Deposited By: PhD Szymon Kubala
Deposited On:09 Nov 2018 09:16
Last Modified:09 Nov 2018 09:16

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