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Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma.

Szmidt, Maciej and Stankiewicz, Adrian and Urbańska, Kaja and Jaworski, Sławomir and Kutwin, Marta and Wierzbicki, Mateusz and Grodzik, Marta and Burzynska, Beata and Gora, Monika and Chwalibog, Andre and Sawosz, Ewa (2019) Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma. BMC Molecular Biology, 20 . p. 2. ISSN 1471-2199

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Official URL: https://bmcmolbiol.biomedcentral.com/articles/10.1...

Abstract

BACKGROUND: Recently different forms of nanographene were proposed as the material with high anticancer potential. However, the mechanism of the suppressive activity of the graphene on cancer development remains unclear. We examined the effect of oxygenated, reduced and pristine graphene on the gene expression in glioblastoma U87 cell line. RESULTS: Conducting microarrays and RT-qPCR analysis we explored that graphene oxide (rather than reduced graphene oxide and pristine graphene) down-regulates the mRNA expression of mitochondrial oxidative phosphorylation (OXPHOS) nuclear genes of complexes I, III, IV and V. The presented results provide first evidence for the hypothesis that the suppressed growth of GBM can be the consequence of down-regulation of OXPHOS protein expression and decreased ATP level. CONCLUSIONS: We suggest that changes in the expression of OXPHOS genes identified in our study may mediate the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells. However, further investigations with different cell lines, regarding expression, regulation and activity of OXPHOS genes identified in our study is necessary to elucidate the mechanism mediating the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Genetics
ID Code:1696
Deposited By: dr Monika Góra
Deposited On:07 Mar 2019 13:47
Last Modified:07 Mar 2019 13:47

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