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Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals

Cholbinski, Piotr and Jastrzebska, Zaneta and Wysocka-Kapcinska, Monika and Plochocka, Danuta and Gornicka, Agnieszka and Hopper, Anita K. and Zoladek, Teresa (2011) Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals. European journal of cell biology, 90 (10). pp. 834-843. ISSN 1618-1298

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Official URL: http://www.elsevier.com/wps/find/journaldescriptio...

Abstract

The Rsp5 ubiquitin ligase regulates numerous cellular processes. Rsp5 is mainly localized to the cytoplasm but nuclear localization was also reported. A potential nuclear export signal was tested for activity by using a GFP2 reporter. The 687-LIGGIAEIDI-696 sequence located in the Hect domain was identified as a nuclear export signal active in a Crm1-dependent manner, and its importance for the localization of Rsp5 was documented by using fluorescence microscopy and a lacZ-based reporter system. Analysis of the cellular location of other Rsp5 fragments fused with GFP2 indicated two independent potential nuclear localization signals, both located in the Hect domain. We also uncovered Rsp5 fragments that are important to targeting/tethering Rsp5 to various regions in the cytoplasm. The presented data indicate that Rsp5 ligase is a shuttling protein whose distribution within the cytoplasm and partitioning between cytoplasmic and nuclear locations is determined by a balance between the actions of several targeting sequences and domains.

Item Type:Article
Uncontrolled Keywords:yeast; Rsp5 ubiquitin ligase; nuclear-cytoplasmic transport
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Genetics
ID Code:170
Deposited By: Prof.dr hab. Teresa Zoladek
Deposited On:29 Sep 2011 09:45
Last Modified:28 Mar 2012 20:36

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