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DNA polymerase ι is acetylated in response to SN2 alkylating agents.

McIntyre, Justyna and Sobolewska, Aleksandra and Fedorowicz, Mikołaj and McLenigan, Mary P and Macias, Matylda and Woodgate, Roger and Sledziewska-Gojska, Ewa (2019) DNA polymerase ι is acetylated in response to SN2 alkylating agents. Scientific Reports, 9 (1). p. 4789. ISSN 2045-2322

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Official URL: https://www.nature.com/articles/s41598-019-41249-3

Abstract

DNA polymerase iota (Polι) belongs to the Y-family of DNA polymerases that are involved in DNA damage tolerance through their role in translesion DNA synthesis. Like all other Y-family polymerases, Polι interacts with proliferating cell nuclear antigen (PCNA), Rev1, ubiquitin and ubiquitinated-PCNA and is also ubiquitinated itself. Here, we report that Polι also interacts with the p300 acetyltransferase and is acetylated. The primary acetylation site is K550, located in the Rev1-interacting region. However, K550 amino acid substitutions have no effect on Polι's ability to interact with Rev1. Interestingly, we find that acetylation of Polι significantly and specifically increases in response to SN2 alkylating agents and to a lower extent to SN1 alkylating and oxidative agents. As we have not observed acetylation of Polι's closest paralogue, DNA polymerase eta (Polη), with which Polι shares many functional similarities, we believe that this modification might exclusively regulate yet to be determined, and separate function(s) of Polι.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Laboratory of Mutagenesis and DNA Repair
ID Code:1711
Deposited By: dr Justyna McIntyre
Deposited On:02 Apr 2019 14:29
Last Modified:19 Feb 2020 14:30

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