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Phosphoproteomic Analysis Reveals that Dehydrins ERD10 and ERD14 are Phosphorylated by SNF1-related Protein Kinase 2.10 in Response to Osmotic Stress

Maszkowska, Justyna and Dębski, Janusz and Kulik, Anna and Kistowski, Michal and Bucholc, Maria and Lichocka, Małgorzata and Klimecka, Maria and Sztatelman, Olga and Szymańska, K and Dadlez, Michal and Dobrowolska, Grażyna (2019) Phosphoproteomic Analysis Reveals that Dehydrins ERD10 and ERD14 are Phosphorylated by SNF1-related Protein Kinase 2.10 in Response to Osmotic Stress. Plant Cell and Environment, 42 (3). pp. 931-946.

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Official URL: https://onlinelibrary.wiley.com/doi/full/10.1111/p...

Abstract

SNF1-related protein kinases 2 (SnRK2s) regulate the plant responses to abiotic stresses, especially water deficits. They are activated in plants subjected to osmotic stress, and some of them are additionally activated in response to enhanced concentrations of abscisic acid (ABA) in plant cells. The SnRK2s that are activated in response to ABA are key elements of ABA signaling that regulate plant acclimation to environmental stresses and ABA-dependent development. Much less is known about the SnRK2s that are not activated by ABA, albeit several studies have shown that these kinases are also involved in response to osmotic stress. Here, we show that one of the Arabidopsis thaliana ABA-non-activated SnRK2s, SnRK2.10, regulates not only the response to salinity but also the plant sensitivity to dehydration. Several potential SnRK2.10 targets phosphorylated in response to stress were identified by a phosphoproteomic approach, including the dehydrins ERD10 and ERD14. Their phosphorylation by SnRK2.10 was confirmed in vitro. Our data suggest that the phosphorylation of ERD14 within the S-segment is involved in the regulation of dehydrin subcellular localization in response to stress.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QK Botany
Divisions:Department of Plant Biochemistry
ID Code:1715
Deposited By: Dr Grażyna Dobrowolska
Deposited On:07 May 2019 09:04
Last Modified:21 Oct 2019 22:05

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