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Poly-saturated dolichols from filamentous fungi modulate activity of dolichol-dependent glycosyltransferase and physical properties of membranes.

Gryz, Elżbieta and Perlinska-Lenart, Urszula and Gawarecka, Katarzyna and Jóźwiak, Adam and Piłsyk, Sebastian and Lipko, Agata and Jemiola-Rzeminska, Małgorzata and Bernat, Przemysław and Muszewska, Anna and Steczkiewicz, Kamil and Ginalski, Krzysztof and Długoński, Jerzy and Strzałka, Kazimierz and Swiezewska, Ewa and Kruszewska, Joanna S. (2019) Poly-saturated dolichols from filamentous fungi modulate activity of dolichol-dependent glycosyltransferase and physical properties of membranes. International Journal of Molecular Sciences, 20 . p. 3043. ISSN 1422-0067

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Official URL: https://www.mdpi.com/1422-0067/20/12/3043

Abstract

Mono-saturated polyprenols (dolichols) have been found in almost all Eukaryotic cells, however, dolichols containing additional saturated bonds at the ω-end, have been identified in A. fumigatus and A. niger. Here, we confirm, using an LC-ESI-QTOF-MS analysis that poly-saturated dolichols are abundant in other filamentous fungi, Trichoderma reesei, A. nidulans and Neurospora crassa, while the yeast Saccharomyces cerevisiae only contains the typical mono-saturated dolichols. We also show, using differential scanning calorimetry (DSC) and fluorescence anisotropy of 1,6-diphenyl-l,3,5-hexatriene (DPH) that the structure of dolichols modulates the properties of membranes and affects the functioning of dolichyl diphosphate mannose synthase (DPMS). The activity of this enzyme from T. reesei and S. cerevisiae was strongly affected by the structure of dolichols. Also the structure of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) model membranes was more strongly disturbed by the poly-saturated dolichols from Trichoderma than by the mono-saturated dolichols from yeast. By comparing the lipidome of filamentous fungi with that from S. cerevisiae we revealed significant differences in the PC/PE ratio and fatty acids composition. Filamentous fungi differ from S. cerevisiae in the lipid composition of their membranes and the structure of dolichols. The structure of dolichols profoundly affects the functioning of dolichol-dependent enzyme, DPMS.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Laboratory of Fungal Glycobiology
ID Code:1726
Deposited By: prof dr hab Joanna S. Kruszewska
Deposited On:27 Jun 2019 08:35
Last Modified:27 Jun 2019 08:35

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