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Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana

Surowiecki, Przemysław and Onyśk, Agnieszka and Mańko, Katarzyna and Swiezewska, Ewa and Surmacz, Liliana (2019) Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana. Molecules, 24 (15). E2789. ISSN 1420-3049

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Official URL: https://www.mdpi.com/1420-3049/24/15/2789

Abstract

Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2Δ or srt1Δ mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue.

Item Type:Article
Subjects:Q Science > QK Botany
Divisions:Department of Lipid Biochemistry
ID Code:1739
Deposited By: dr Liliana Surmacz
Deposited On:19 Aug 2019 11:33
Last Modified:19 Aug 2019 11:33

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