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New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination

Lechowska, Katarzyna and Kubala, Szymon and Wojtyla, Łukasz and Nowaczyk, Grzegorz and Quinet, Muriel and Lutts, Stanley and Garnczarska, Małgorzata (2019) New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination. International Journal of Molecular Sciences, 20 (3). pp. 1-24. ISSN 1422-0067

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Official URL: https://www.mdpi.com/1422-0067/20/3/540

Abstract

Seed priming is a pre-sowing method successfully used to improve seed germination. Since water plays a crucial role in germination, the aim of this study was to investigate the relationship between better germination performances of osmoprimed Brassica napus seeds and seed water status during germination. To achieve this goal, a combination of different kinds of approaches was used, including nuclear magnetic resonance (NMR) spectroscopy, TEM, and SEM as well as semi-quantitative PCR (semi-qPCR). The results of this study showed that osmopriming enhanced the kinetics of water uptake and the total amount of absorbed water during both the early imbibition stage and in the later phases of seed germination. The spin–spin relaxation time (T2) measurement suggests that osmopriming causes faster water penetration into the seed and more efficient tissue hydration. Moreover, factors potentially affecting water relations in germinating primed seeds were also identified. It was shown that osmopriming (i) changes the microstructural features of the seed coat, e.g., leads to the formation of microcracks, (ii) alters the internal structure of the seed by the induction of additional void spaces in the seed, (iii) increases cotyledons cells vacuolization, and (iv) modifies the expression pattern of aquaporin genes.

Item Type:Article
Uncontrolled Keywords:seed priming; germination; water status; aquaporin genes; NMR spectroscopy; SEM microscopy; TEM microscopy
Subjects:Q Science > QK Botany
Q Science > QP Physiology
Divisions:Department of Protein Biosynthesis
ID Code:1824
Deposited By: PhD Szymon Kubala
Deposited On:03 Feb 2020 12:56
Last Modified:03 Feb 2020 12:56

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