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Polymerase iota - an odd sibling among Y family polymerases

McIntyre, Justyna (2020) Polymerase iota - an odd sibling among Y family polymerases. DNA Repair, 86 (102753). ISSN 1568-7864

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Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

It has been two decades since the discovery of the most mutagenic human DNA polymerase, polymerase iota (Polι). Since then, the biochemical activity of this translesion synthesis (TLS) enzyme has been extensively explored, mostly through in vitro experiments, with some insight into its cellular activity. Polι is one of four members of the Y-family of polymerases, which are the best characterized DNA damage-tolerant polymerases involved in TLS. Polι shares some common Y-family features, including low catalytic efficiency and processivity, high infidelity, the ability to bypass some DNA lesions, and a deficiency in 3'→5' exonucleolytic proofreading. However, Polι exhibits numerous properties unique among the Y-family enzymes. Polι has an unusual catalytic pocket structure and prefers Hoogsteen over Watson-Crick pairing, and its replication fidelity strongly depends on the template; further, it prefers Mn2+ ions rather than Mg2+ as catalytic activators. In addition to its polymerase activity, Polι possesses also 5'-deoxyribose phosphate (dRP) lyase activity, and its ability to participate in base excision repair has been shown. As a highly error-prone polymerase, its regulation is crucial and mostly involves posttranslational modifications and protein-protein interactions. The upregulation and downregulation of Polι are correlated with different types of cancer and suggestions regarding the possible function of this polymerase have emerged from studies of various cancer lines. Nonetheless, after twenty years of research, the biological function of Polι  certainly remains unresolved.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions:Laboratory of Mutagenesis and DNA Repair
ID Code:1848
Deposited By: dr Justyna McIntyre
Deposited On:19 Feb 2020 14:28
Last Modified:19 Feb 2020 14:28

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