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Response to a DNA vaccine against the H5N1 virus depending on the chicken line and number of doses

Kalenik, Barbara Małgorzata and Góra-Sochacka, Anna and Stachyra, Anna and Olszewska-Tomczyk, Monika and Fogtman, Anna and Sawicka, Róża and Śmietanka, Krzysztof and Sirko, Agnieszka (2020) Response to a DNA vaccine against the H5N1 virus depending on the chicken line and number of doses. Virology Journal, 17 (1). ISSN 1743-422X


Official URL: http://doi.org/10.1186/s12985-020-01335-9


Background Avian influenza virus infections cause significant economic losses on poultry farms and pose the threat of a possible pandemic outbreak. Routine vaccination of poultry against avian influenza is not recommended in Europe, however it has been ordered in some other countries, and more countries are considering use of the avian influenza vaccine as a component of their control strategy. Although a variety of such vaccines have been tested, most research has concentrated on specific antibodies and challenge experiments. Methods We monitored the transcriptomic response to a DNA vaccine encoding hemagglutinin from the highly pathogenic H5N1 avian influenza virus in the spleens of broiler and layer chickens. Moreover, in layer chickens the response to one and two doses of the vaccine was compared. Results All groups of birds immunized with two doses of the vaccine responded at the humoral level by producing specific anti-hemagglutinin antibodies. A response to the vaccine was also detected in the spleen transcriptomes. Differential expression of many genes encoding noncoding RNA and proteins functionally connected to the neuroendocrine-immune system was observed in different immunized groups.

Item Type:Article
Subjects:Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
ID Code:1872
Deposited By: dr Anna Gora-Sochacka
Deposited On:11 May 2020 08:54
Last Modified:11 May 2020 08:54

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