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Plasmidome of an environmental Acinetobacter lwoffii strain originating from a former gold and arsenic mine

Walter, Tomasz and Klim, Joanna and Jurkowski, Marcin and Gawor, Jan and Köhling, Iwona and Słodownik, Małgorzata and Zielenkiewicz, Urszula (2020) Plasmidome of an environmental Acinetobacter lwoffii strain originating from a former gold and arsenic mine. Plasmid, 110 . pp. 1-17. ISSN 0147-619X

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Abstract

Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids of different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry a diverse number of genes, allowing the host cell to survive in an environment that would otherwise be lethal or restrictive for growth. The present study characterizes the plasmidome generated from an arsenic-resistant strain named ZS207, classified as Acinetobacter lwoffii . Sequencing effort revealed the presence of nine plasmids in the size between 4.3 and 38.4 kb as well as one 186.6 kb megaplasmid. All plasmids, except the megaplasmid, do apparently not confer distinguishing phenotypic features . In contrast, the megaplasmid carries arsenic and heavy metals resistance regions similar to those found in permafrost A. lwoffii strains. In-depth in silico analyses have shown a significant similarity between the regions from these plasmids, especially concerning multiple transposable elements, transfer and mobilization genes, and toxin-antitoxin systems. Since ars genes encode proteins of major significance in terms of potential use in bioremediation, arsenic resistance level of ZS207 was determined and the functionality of selected ars genes was examined . Additionally, we checked the functionality of plasmid-encoded toxin-antitoxin systems and their impact on the formation of persister cells.

Item Type:Article
Subjects:Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:1904
Deposited By: Dr. Urszula Zielenkiewicz
Deposited On:30 Jul 2020 07:12
Last Modified:30 Jul 2020 07:12

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