IBB PAS Repository

Biodiversity and habitats of polar region polyhydroxyalkanoic acid-producing bacteria: bioprospection by popular screening methods

Rogala, Małgorzata Marta and Gawor, Jan and Gromadka, Robert and Kowalczyk, Magdalena and Grzesiak, Jakub (2020) Biodiversity and habitats of polar region polyhydroxyalkanoic acid-producing bacteria: bioprospection by popular screening methods. Genes, 11 (8). pp. 873-890. ISSN 2073-4425

[img] PDF
1MB

Official URL: https://www.mdpi.com/2073-4425/11/8/873

Abstract

Polyhydroxyalkanoates (PHA), the intracellular polymers produced by various microorganisms as carbon and energy storage, are of great technological potential as biodegradable versions of common plastics. PHA-producing microbes are therefore in great demand and a plethora of different environments, especially extreme habitats, have been probed for the presence of PHA-accumulators. However, polar region have been neglected in this regard, probably due to the low accessibility of the sampling material and unusual cultivation regime. Here, we present the results of a screening procedure involving 200 bacterial strains isolated 25 habitats of both polar regions. Agar-based tests, microscopy and genetic methods were conducted to elucidate the biodiversity and potential of polar-region PHA-accumulators. Microscopic observation of Nile Red stained cells proved to be the most reliable screening method as it allowed to confirm the characteristic bright orange glow of the Nile Red – PHA complex as well as the typical morphology of the PHA inclusions. Psychrophilic PHA-producers belonged mostly to the Comamonadaceae family (Betaproteobacteria) although actinobacterial PHA synthesizers of the families Microbacteriaceae and Micrococcaceae also featured prominently. Glacial and postglacial habitats as well as developed polar region soils were evaluated as promising for PHA-producer bioprospection. This study highlights the importance of psychrophiles as biodiverse and potent polyhydroxyalkanote sources for scientific and application-aimed research.

Item Type:Article
Subjects:Q Science > QR Microbiology
Divisions:Department of Antarctic Biology
Department of Microbial Biochemistry
Laboratory of DNA Sequencing and Oligonucleotide Synthesis
ID Code:1905
Deposited By: Jakub Grzesiak
Deposited On:04 Aug 2020 07:36
Last Modified:04 Aug 2020 07:36

Repository Staff Only: item control page