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Transcriptional Organization of the Stability Module of BroadHost-Range Plasmid RA3, from the IncU Group

Lewicka, Ewa and Dolowy, Patrycja and Godziszewska, Jolanta and Litwin, Emilia and Ludwiczak, Marta and Jagura-Burdzy, Grazyna (2020) Transcriptional Organization of the Stability Module of BroadHost-Range Plasmid RA3, from the IncU Group. Applied and Environmental Microbiology, 86 (3). e00847-20. ISSN 0099-2240

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Official URL: https://aem.asm.org/content/early/2020/06/10/AEM.0...

Abstract

The broad-host-range (BHR) conjugative plasmids have developed diverse adaptive mechanisms defining the range of their promiscuity. The BHR conjugative RA3 plasmid, the archetype of the IncU group, can transfer between, replicate in, and be maintained in representatives of Alpha-, Beta-, and Gammaproteobacteria. Its stability module encompasses ten open reading frames (ORFs) apparently organized into five operons, all transcribed in the same direction from several strong promoters that are tightly regulated either by autorepressors or by global plasmidencoded regulators. In this paper, we demonstrate that owing to an efficient RNA polymerase (RNAP) read-through, the transcription from the first promoter, orf02p, may continue through the whole module. Moreover, an analysis of mRNA produced from the wild-type (WT) stability module and its deletion variants deprived of particular internal transcription initiation sites reveals that in fact each operon may be transcribed from any upstream promoter, giving rise to multicistronic transcripts of variable length and creating an additional level of gene expression control by transcript dosage adjustment. The gene expression patterns differ among various hosts, indicating that promoter recognition, regulation, and the RNAP read-through mechanisms are modulated in a species-specific manner.

Item Type:Article
Uncontrolled Keywords:RNAP read-through, broad-host-range plasmid, gene expression, stability functions, transcript dosage
Subjects:Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:1914
Deposited By: prof Grazyna Jagura-Burdzy
Deposited On:19 Aug 2020 12:46
Last Modified:19 Aug 2020 12:46

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