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Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.)

Perlinska-Lenart, Urszula and Piłsyk, Sebastian and Gryz, Elzbieta and Turło, Jadwiga and Hilszczańska, Dorota and Kruszewska, Joanna S. (2020) Identification of bacteria and fungi inhabiting fruiting bodies of Burgundy truffle (Tuber aestivum Vittad.). Archives of Microbiology .

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Official URL: https://link.springer.com/article/10.1007/s00203-0...

Abstract

Tuber species may be regarded as complex microhabitats hosting diverse microorganisms inside their fruiting bodies. Here, we investigated the structure of microbial communities inhabiting the gleba of wild growing (in stands) T. aestivum, using Illumina sequencing and culture-based methods. The two methods used in combination allowed to extract more information on complex microbiota of Tuber aestivum gleba. Analysis of the V3–V4 region of 16S rDNA identified nine phyla of bacteria present in the gleba of T. aestivum ascomata, mostly Proteobacteria from the family Bradyrhizobiaceae. Our results ideally match the earlier data for other Tuber species where the family Bradyrhizobiaceae was the most represented. The ITS1 region of fungal rDNA represented six alien fungal species belonging to three phyla. To complement the metagenomic analysis, cultivable fungi and bacteria were obtained from the gleba of the same T. aestivum fruiting bodies. The identified fungi mostly belong to the phylum Basidiomycota and same to Ascomycota. Analysis of cultivable bacteria revealed that all the specimens were colonized by different strains of Bacillus. Fungal community inhabiting T. aestivum fruiting bodies was never shown before.

Item Type:Article
Subjects:Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions:Laboratory of Fungal Glycobiology
ID Code:1918
Deposited By: prof dr hab Joanna S. Kruszewska
Deposited On:02 Sep 2020 11:18
Last Modified:02 Sep 2020 11:18

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