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Slow Adaptive Response of Budding Yeast Cells to Stable Conditions of Continuous Culture Can Occur without Genome Modifications

Klim, Joanna and Zielenkiewicz, Urszula and Kurlandzka, Anna and Kaczanowski, Szymon and Skoneczny, Marek (2020) Slow Adaptive Response of Budding Yeast Cells to Stable Conditions of Continuous Culture Can Occur without Genome Modifications. Genes, 11 (12). p. 1419. ISSN 2073-4425


Official URL: https://www.mdpi.com/2073-4425/11/12/1419


Continuous cultures assure the invariability of environmental conditions and the metabolic state of cultured microorganisms, whereas batch-cultured cells undergo constant changes in nutrients availability. For that reason, continuous culture is sometimes employed in the whole transcriptome, whole proteome, or whole metabolome studies. However, the typical method for establishing uniform growth of a cell population, i.e., by limited chemostat, results in the enrichment of the cell population gene pool with mutations adaptive for starvation conditions. These adaptive changes can skew the results of large-scale studies. It is commonly assumed that these adaptations reflect changes in the genome, and this assumption has been confirmed experimentally in rare cases. Here we show that in a population of budding yeast cells grown for over 200 generations in continuous culture in non-limiting minimal medium and therefore not subject to selection pressure, remodeling of transcriptome occurs, but not as a result of the accumulation of adaptive mutations. The observed changes indicate a shift in the metabolic balance towards catabolism, a decrease in ribosome biogenesis, a decrease in general stress alertness, reorganization of the cell wall, and transactions occurring at the cell periphery. These adaptive changes signify the acquisition of a new lifestyle in a stable nonstressful environment. The absence of underlying adaptive mutations suggests these changes may be regulated by another mechanism.

Item Type:Article
Subjects:Q Science > QH Natural history > QH426 Genetics
Divisions:Department of Bioinformatics
Department of Genetics
Department of Microbial Biochemistry
ID Code:1957
Deposited By: Dr hab. Marek Skoneczny
Deposited On:05 Jan 2021 08:07
Last Modified:05 Jan 2021 08:07

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