IBB PAS Repository

Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis

Rojek, Joanna and Tucker, Matthew R. and Pinto, Sara C. and Rychłowski, Michał and Lichocka, Małgorzata and Soukupova, Hana and Nowakowska, Julita and Bohdanowicz, Jerzy and Surmacz, Gabriela and Gutkowska, Malgorzata (2021) Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis. Journal of Experimental Botany, 72 (2). pp. 320-340. ISSN 0022-0957


Official URL: https://academic.oup.com/jxb/article/72/2/320/5906...


Eukaryotic cells rely on the accuracy and efficiency of vesicular traffic. In plants, disturbances in vesicular trafficking are well studied in quickly dividing root meristem cells or polar growing root hairs and pollen tubes. The development of the female gametophyte, a unique haploid reproductive structure located in the ovule, has received far less attention in studies of vesicular transport. Key molecules providing the specificity of vesicle formation and its subsequent recognition and fusion with the acceptor membrane are Rab proteins. Rabs are anchored to membranes by covalently linked geranylgeranyl group(s) that are added by the Rab geranylgeranyl transferase (RGT) enzyme. Here we show that Arabidopsis plants carrying mutations in the gene encoding the β-subunit of RGT (rgtb1) exhibit severely disrupted female gametogenesis and this effect is of sporophytic origin. Mutations in rgtb1 lead to internalization of the PIN1 and PIN3 proteins from the basal membranes to vesicles in provascular cells of the funiculus. Decreased transport of auxin out of the ovule is accompanied by auxin accumulation in tissue surrounding the growing gametophyte. In addition, female gametophyte development arrests at the uni- or binuclear stage in a significant portion of the rgtb1 ovules. These observations suggest that communication between the sporophyte and the developing female gametophyte relies on Rab-dependent vesicular traffic of the PIN1 and PIN3 transporters and auxin efflux out of the ovule.

Item Type:Article
Subjects:Q Science > QK Botany
Divisions:Department of Lipid Biochemistry
ID Code:2004
Deposited By: PhD Malgorzata Lichocka
Deposited On:22 Mar 2021 10:35
Last Modified:23 Mar 2021 07:42

Repository Staff Only: item control page