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Proteomic Profile of Saliva in Parkinson’s Disease Patients: A Proof of Concept Study

1
Department of Neurology, Faculty of Health Sciences, Medical University of Warsaw, 03-242 Warsaw, Poland
2
Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Manuel Menéndez González
Brain Sci. 2021, 11(5), 661; https://doi.org/10.3390/brainsci11050661
Received: 10 April 2021 / Revised: 11 May 2021 / Accepted: 13 May 2021 / Published: 18 May 2021
(This article belongs to the Section Neurodegenerative Diseases)
Parkinson’s disease (PD) is a progressive neurodegenerative disorder. It affects many organs. Lewy bodies—a histopathological “hallmark” of PD—are detected in about 75% of PD submandibular gland samples. We hypothesize that saliva can be a source of biomarkers of PD. The aim of the study was to evaluate and compare the salivary proteome of PD patients and healthy controls (HC). Salivary samples from 39 subjects (24 PD patients, mean age 61.6 ± 8.2; 15 HC, mean age 60.9 ± 6.7) were collected. Saliva was collected using RNA-Pro-Sal kits. Label-free LC-MS/MS mass spectrometry was performed to characterize the proteome of the saliva. IPA analysis of upstream inhibitors was performed. A total of 530 proteins and peptides were identified. We observed lower concentrations of S100-A16, ARP2/3, and VPS4B in PD group when compared to HC. We conclude that the salivary proteome composition of PD patients is different than that of healthy controls. We observed a lower concentration of proteins involved in inflammatory processes, exosome formation, and adipose tissue formation. The variability of expression of proteins between the two groups needs to be considered. View Full-Text
Keywords: Parkinson’s disease; saliva; proteomic profile; neurodegeneration; salivary glands Parkinson’s disease; saliva; proteomic profile; neurodegeneration; salivary glands
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MDPI and ACS Style

Figura, M.; Sitkiewicz, E.; Świderska, B.; Milanowski, Ł.; Szlufik, S.; Koziorowski, D.; Friedman, A. Proteomic Profile of Saliva in Parkinson’s Disease Patients: A Proof of Concept Study. Brain Sci. 2021, 11, 661. https://doi.org/10.3390/brainsci11050661

AMA Style

Figura M, Sitkiewicz E, Świderska B, Milanowski Ł, Szlufik S, Koziorowski D, Friedman A. Proteomic Profile of Saliva in Parkinson’s Disease Patients: A Proof of Concept Study. Brain Sciences. 2021; 11(5):661. https://doi.org/10.3390/brainsci11050661

Chicago/Turabian Style

Figura, Monika, Ewa Sitkiewicz, Bianka Świderska, Łukasz Milanowski, Stanisław Szlufik, Dariusz Koziorowski, and Andrzej Friedman. 2021. "Proteomic Profile of Saliva in Parkinson’s Disease Patients: A Proof of Concept Study" Brain Sciences 11, no. 5: 661. https://doi.org/10.3390/brainsci11050661

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