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Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae

Tudek, Agnieszka and Krawczyk, Pawel S and Mroczek, Seweryn and Tomecki, Rafał and Turtola, Matti Ari and Matylla-Kulińska, Katarzyna and Jensen, Torben H. and Dziembowski, Andrzej (2021) Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae. Nature Communications, 12 . ISSN 2041-1723

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Official URL: https://www.nature.com/articles/s41467-021-25251-w


The polyadenosine tail (poly[A]-tail) is a universal modification of eukaryotic messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). In budding yeast, Pap1-synthesized mRNA poly(A) tails enhance export and translation, whereas Trf4/5-mediated polyadenylation of ncRNAs facilitates degradation by the exosome. Using direct RNA sequencing, we decipher the extent of poly(A) tail dynamics in yeast defective in all relevant exonucleases, deadenylases, and poly(A) polymerases. Predominantly ncRNA poly(A) tails are 20-60 adenosines long. Poly(A) tails of newly transcribed mRNAs are 50 adenosine long on average, with an upper limit of 200. Exonucleolysis by Trf5-assisted nuclear exosome and cytoplasmic deadenylases trim the tails to 40 adenosines on average. Surprisingly, PAN2/3 and CCR4-NOT deadenylase complexes have a large pool of non-overlapping substrates mainly defined by expression level. Finally, we demonstrate that mRNA poly(A) tail length strongly responds to growth conditions, such as heat and nutrient deprivation.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
ID Code:2081
Deposited By: Dr Agnieszka Tudek
Deposited On:01 Sep 2021 14:44
Last Modified:01 Sep 2021 14:44

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