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The Lrp/AsnC-Type Regulator PA2577 Controls the EamA-Like Transporter Gene PA2576 in Pseudomonas aeruginosa

Modrzejewska, Magdalena and Kawalek, Adam and Bartosik, Aneta A (2021) The Lrp/AsnC-Type Regulator PA2577 Controls the EamA-Like Transporter Gene PA2576 in Pseudomonas aeruginosa. International Journal of Molecular Sciences, 22 (13340). ISSN 1422-0067


Official URL: https://www.mdpi.com/1422-0067/22/24/13340


The regulatory network of gene expression in Pseudomonas aeruginosa, an opportunistic human pathogen, is very complex. In the PAO1 reference strain, about 10% of genes encode transcriptional regulators, many of which have undefined regulons and unknown functions. The aim of this study is the characterization of PA2577 protein, a representative of the Lrp/AsnC family of transcriptional regulators. This family encompasses proteins involved in the amino acid metabolism, regulation of transport processes or cell morphogenesis. The transcriptome profiling of P. aeruginosa cells with mild PA2577 overproduction revealed a decreased expression of the PA2576 gene oriented divergently to PA2577 and encoding an EamA-like transporter. A gene expression analysis showed a higher mRNA level of PA2576 in P. aeruginosa DPA2577, indicating that PA2577 acts as a repressor. Concomitantly, ChIP-seq and EMSA assays confirmed strong interactions of PA2577 with the PA2577/PA2576 intergenic region. Additionally, phenotype microarray analyses indicated an impaired metabolism of DPA2576 and DPA2577 mutants in the presence of polymyxin B, which suggests disturbances of membrane functions in these mutants. We show that PA2576 interacts with two proteins, PA5006 and PA3694, with a predicted role in lipopolysaccharide (LPS) and membrane biogenesis. Overall, our results indicate that PA2577 acts as a repressor of the PA2576 gene coding for the EamA-like transporter and may play a role in the modulation of the cellular response to stress conditions, including antimicrobial peptides, e.g., polymyxin B.

Item Type:Article
Uncontrolled Keywords:Pseudomonas aeruginosa; Lrp/AsnC transcriptional regulators; RNA-seq; ChIP-seq; PA2577 regulon; EamA-like transporter
Subjects:Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:2122
Deposited By: PhD AA Bartosik
Deposited On:15 Dec 2021 08:03
Last Modified:15 Dec 2021 08:03

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