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Synthesis of Dolichols in Candida albicans is Co-Regulated With Elongation of Fatty Acids

Janik, Anna and Perlinska-Lenart, Urszula and Gawarecka, Katarzyna and Augustyniak, Justyna and Bratek -Gerej, Ewelina and Bernat, Przemysław and Piłsyk, Sebastian and Skalmowska, Patrycja and Swiezewska, Ewa and Kruszewska, Joanna S. (2022) Synthesis of Dolichols in Candida albicans is Co-Regulated With Elongation of Fatty Acids. International Journal of Molecular Sciences, 23 . ISSN 1422-0067


Official URL: https://www.mdpi.com/1422-0067/23/1/409


Abstract: Protein glycosylation requires dolichyl phosphate as a carbohydrate carrier. Dolichols are α-saturated polyprenols, and their saturation in S. cerevisiae is catalyzed by polyprenyl re-ductase Dfg10 together with some other unknown enzymes. The aim of this study was to identi-fy such enzymes in Candida. The Dfg10 polyprenyl reductase from S. cerevisiae comprises a C-terminal 3-oxo-5-alpha-steroid 4-dehydrogenase domain. Alignment analysis revealed such a domain in two ORFs (orf19.209 and orf19.3293) from C. albicans, which were similar, respective-ly, to Dfg10 polyprenyl reductase and Tsc13 enoyl-transferase from S. cerevisiae. Deletion of orf19.209 in Candida impaired saturation of polyprenols. The Tsc13 homologue turned out not to be capable of saturating polyprenols, but limiting its expression reduce the cellular level of dol-ichols and polyprenols. This reduction was not due to a decreased expression of genes encoding cis-prenyltransferases from the dolichol branch but to a lower expression of genes encoding en-zymes of the early stages of the mevalonate pathway. Despite the resulting lower consumption of acetyl-CoA, the sole precursor of the mevalonate pathway, it was not redirected towards fatty acid synthesis or elongation. Lowering the expression of TSC13 decreased the expression of the ACC1 gene encoding acetyl-CoA carboxylase, the key regulatory enzyme of fatty acid synthesis and elongation.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Laboratory of Fungal Glycobiology
ID Code:2135
Deposited By: prof dr hab Joanna S. Kruszewska
Deposited On:24 Jan 2022 11:21
Last Modified:24 Jan 2022 11:21

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