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cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis

Gawarecka, Katarzyna and Siwinska, Joanna and Poznański, Jarosław and Onyśk, Agnieszka and Surowiecki, Przemysław and Sztompka, Karolina and Surmacz, Liliana and Ahn, Ji Hoon and Korte, Arthur and Swiezewska, Ewa and Ihnatowicz, Anna (2022) cis‐prenyltransferase 3 and α/β‐hydrolase are new determinants of dolichol accumulation in Arabidopsis. Plant, cell & environment, 45 (2). pp. 479-495. ISSN 1365-3040

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Official URL: https://onlinelibrary.wiley.com/doi/10.1111/pce.14...

Abstract

Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for posttranslational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis‐prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β‐hydrolase—is experimentally confirmed. These data will help to generate Dol‐enriched plants which might serve as a remedy for Dol‐deficiency in humans.

Item Type:Article
Uncontrolled Keywords:dolichol, GWAS, isoprenoid, natural variation, plant‐environment interactions, polyprenol, QTL mapping, secondary metabolism
Subjects:Q Science > Q Science (General)
Q Science > QP Physiology
Divisions:Department of Lipid Biochemistry
ID Code:2140
Deposited By: dr Liliana Surmacz
Deposited On:15 Feb 2022 15:45
Last Modified:15 Feb 2022 15:45

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