IBB PAS Repository

Efficacy and safety of phage therapy against Salmonella enterica serovars Typhimurium and Enteritidis estimated by using a battery of in vitro tests and the Galleria mellonella animal model

Kosznik-Kwaśnicka, Katarzyna and Stasiłojć, Małgorzata and Grabowski, Łukasz and Zdrojewska, Karolina and Węgrzyn, Grzegorz and Węgrzyn, Alicja (2022) Efficacy and safety of phage therapy against Salmonella enterica serovars Typhimurium and Enteritidis estimated by using a battery of in vitro tests and the Galleria mellonella animal model. Microbiological Research, 261 (8). p. 127052. ISSN 0944-5013

[img] PDF
13MB

Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

In light of spreading antibiotic resistance among pathogenic bacteria, the development of novel approaches to combat such microorganisms is crucial. Salmonella enterica is pathogenic to humans, however, it can also infect poultry, being a potential foodborne pathogen when poultry-derived food is contaminated by this bacterium. Phage therapy is one of the alternative ways to treat Salmonella-infected animals while the establishment of this method and its introduction to a general practice requires detailed studies on safety and efficacy. Here, we present the results of such studies with two previously isolated and characterized bacteriophages, vB_SenM2 and vB_Sen-TO17, and four strains of S. enterica belonging to two serovars, Typhimurium and Enteritidis. We demonstrated effective reduction of bacterial cell number and cell culture density when using each phage alone, and in combination (as a cocktail). These phages were also effective in reducing bacterial biofilm. The efficacy of this in vitro phage therapy was compared to the action of known antibiotics, as was the efficiency of appearance of bacteria resistant to both these types of antibacterial agents. Safety of the use of bacteriophages was demonstrated using the LAL chromogenic test and the chicken fibroblast viability assay. Finally, the efficacy of phage therapy was assessed with the in vivo model of S. enterica-infected Galleria mellonella larvae, showing a significant improvement in the survival of the animals. In conclusion, we demonstrated high efficacy and acceptable safety profiles of phage therapy against S. enterica strains using vB_SenM-2 and vB_Sen-TO17 phages (both alone and in a cocktail). These results open a possibility for a trial with the use of poultry and these phages which might potentially allow to introduce of this method for practical use in poultry farming.

Item Type:Article
Subjects:Q Science > QR Microbiology
Q Science > QR Microbiology > QR355 Virology
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:2151
Deposited By: MSc Łukasz Grabowski
Deposited On:27 May 2022 14:20
Last Modified:27 May 2022 14:20

Repository Staff Only: item control page