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Novel broad-host-range vehicles for cloning and shuffling of gene cassettes

Bartosik, Aneta A and Markowska, Aleksandra and Szarlak, Jolanta and Kulinska, Anna and Jagura-Burdzy, Grazyna (2012) Novel broad-host-range vehicles for cloning and shuffling of gene cassettes. Journal of Microbiological Methods, 88 (1). pp. 53-62.

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Official URL: http://www.elsevier .com/locate/jmicmeth


Novel vectors for cloning and shuffling of gene cassettes based on minireplicon of broadhost- range RA3 plasmid from IncU incompatibility group were constructed. A series of minireplicon variants were prepared with copy number ranging from low (1-2 copies per chromosome), medium (10-15 copies per chromosome) to high copy number (80-90 copies per chromosome). The new cloning vectors are relatively small in size (4.5-5.4 kb) and carry various resistance determinants: kanamycin (KmR), tetracycline (TcR) or chloramphenicol (CmR). The vectors were engineered to facilitate cloning and shuffling of the functional modules with or without transcriptional terminators. Using the described strategy, a bank of functional modules, ready for exchange, has been initiated.

Item Type:Article
Uncontrolled Keywords:IncU minireplicon New biotechnology tools Library of functional modules Cassette shuffling
Subjects:Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:220
Deposited By: prof Grazyna Jagura-Burdzy
Deposited On:15 Mar 2012 08:18
Last Modified:15 Jan 2015 14:02

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