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Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa

Kawalek, Adam and Glabski, Krzysztof and Bartosik, Aneta A and Wozniak, Dominika and Kusiak, Magdalena and Gawor, Jan and Zuchniewicz, Karolina and Jagura-Burdzy, Grazyna (2023) Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa. Microbiology Spectrum, 11 (1). pp. 1-18. ISSN 2165-0497


Official URL: https://journals.asm.org/doi/10.1128/spectrum.0428...


In the majority of bacterial species, the tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its target parS sequence(s), assists in the chromosome partitioning. ParB forms large nucleoprotein complexes at parS(s), located in the vicinity of origin of chromosomal replication (oriC), which after replication are subsequently positioned by ParA in cell poles. Remarkably, ParA and ParB participate not only in the chromosome segregation but through interactions with various cellular partners they are also involved in other cell cycle-related processes, in a species-specific manner. In this work, we characterized Pseudomonas aeruginosa ParB interactions with the cognate ParA, showing that the N-terminal motif of ParB is required for these interactions, and demonstrated that ParAB-parS-mediated rapid segregation of newly replicated ori domains prevented structural maintenance of chromosome (SMC)-mediated cohesion of sister chromosomes. Furthermore, using proteome-wide techniques, we have identified other ParB partners in P. aeruginosa, which encompass a number of proteins, including the nucleoid-associated proteins NdpA(PA3849) and NdpA2, MinE (PA3245) of Min system, and transcriptional regulators and various enzymes, e.g., CTP synthetase (PA3637). Among them are also NTPases PA4465, PA5028, PA3481, and FleN (PA1454), three of them displaying polar localization in bacterial cells. Overall, this work presents the spectrum of P. aeruginosa ParB partners and implicates the role of this protein in the cross-talk between chromosome segregation and other cellular processes.

Item Type:Article
Uncontrolled Keywords:DNA segregation, Pseudomonas aeruginosa, partitioning proteins
Subjects:Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions:Department of Microbial Biochemistry
ID Code:2258
Deposited By: dr Adam Kawałek
Deposited On:06 Mar 2023 13:04
Last Modified:06 Mar 2023 13:04

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