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Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt

Montez, Miguel and Majchrowska, Maria and Krzyszton, Michal and Bokota, Grzegorz and Sacharowski, S.P. and Wrona, Magdalena and Yatusevich, Ruslan and Massana, Ferran and Plewczynski, Dariusz and Świeżewski, Szymon (2023) Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to salt. The EMBO Journal, 42 (5). e112443. ISSN 0261-4189

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Official URL: http://doi.org/10.15252/embj.2022112443

Abstract

Eukaryotic genomes are pervasively transcribed by RNA polymerase II. Yet, the molecular and biological implications of such a phenomenon are still largely puzzling. Here, we describe noncoding RNA transcription upstream of the Arabidopsis thaliana DOG1 gene, which governs salt stress responses and is a key regulator of seed dormancy. We find that expression of the DOG1 gene is induced by salt stress, thereby causing a delay in seed germination. We uncover extensive transcriptional activity on the promoter of the DOG1 gene, which produces a variety of lncRNAs. These lncRNAs, named PUPPIES, are co-directionally transcribed and extend into the DOG1 coding region. We show that PUPPIES RNAs respond to salt stress and boost DOG1 expression, resulting in delayed germination. This positive role of pervasive PUPPIES transcription on DOG1 gene expression is associated with augmented pausing of RNA polymerase II, slower transcription and higher transcriptional burst size. These findings highlight the positive role of upstream co-directional transcription in controlling transcriptional dynamics of downstream genes.

Item Type:Article
Subjects:A General Works > AC Collections. Series. Collected works
ID Code:2305
Deposited By: Unnamed user with email sswiez@ibb.waw.pl
Deposited On:07 Aug 2023 09:04
Last Modified:21 May 2024 11:23

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