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Symmetric and asymmetric DNA N6-adenine methylation regulates different biological responses in Mucorales

Lax, Carlos and Mondo, Stephen and Osorio-Concepcion, Macario and Muszewska, Anna and Corrochano-Luque, Maria and Gutiérrez, Gabriel and Riley, Robert and Lipzen, Anna and Guo, Jie and Hurdley, Hope and Amirebrahimi, Mojgan and Ng, Vivian and Lorenzo-Gutiérrez, Damaris and Binder, Urlike and Yang, Junhuan and Song, Yuanda and Cánovas, David and Navarro, ENavarro and Freitag, Michael and Gabaldon, Toni and Grigoriev, Igor and Corrochano, Luis and Nicolás, Francisco and Garre, Victoriano (2024) Symmetric and asymmetric DNA N6-adenine methylation regulates different biological responses in Mucorales. Nature Communications . ISSN 2041-1723

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Official URL: https://www.nature.com/articles/s41467-024-50365-2

Abstract

DNA N6-adenine methylation (6mA) has recently gained importance as an epigenetic modification in eukaryotes. Its function in lineages with high levels, such as early-diverging fungi (EDF), is of particular interest. Here, we investigated the biological significance and evolutionary implications of 6mA in EDF, which exhibit divergent evolutionary patterns in 6mA usage. The analysis of two Mucorales species displaying extreme 6mA usage reveals that species with high 6mA levels show symmetric methylation enriched in highly expressed genes. In contrast, species with low 6mA levels show mostly asymmetric 6mA. Interestingly, transcriptomic regulation throughout development and in response to environmental cues is associated with changes in the 6mA landscape. Furthermore, we identify an EDF-specific methyltransferase, likely originated from endosymbiotic bacteria, as responsible for asymmetric methylation, while an MTA-70 methylation complex performs symmetric methylation. The distinct phenotypes observed in the corresponding mutants reinforced the critical role of both types of 6mA in EDF.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions:Department of Microbial Biochemistry
ID Code:2431
Deposited By: dr Anna Muszewska
Deposited On:19 Jul 2024 10:53
Last Modified:19 Jul 2024 10:53

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