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A functional study of genes essential for autogamy and nuclear reorganization in Paramecium

Nowak, Jacek K. and Gromadka, Robert and Juszczuk, Marek and Jerka-Dziadosz, Maria and Maliszewska, Kamila and Mucchielli, Marie-Hélène and Gout, Jean-François and Arnaiz, Olivier and Agier, Nicolas and Tang, Thomas and Aggerbeck, Lawrence P. and Cohen, Jean and Delacroix, Hervé and Sperling, Linda and Herbert, Christopher J. and Zagulski, Marek and Bétermier, Mireille (2011) A functional study of genes essential for autogamy and nuclear reorganization in Paramecium. Eukaryotic Cell, 10 (3). ISSN 1535-9786 (In Press)

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Official URL: http://ec.asm.org/


Like all ciliates, Paramecium tetraurelia is a unicellular eukaryote that harbors two kinds of nuclei within its cytoplasm. At each sexual cycle, a new somatic macronucleus (MAC) develops from the germline micronucleus (MIC) through a sequence of complex events, which includes meiosis, karyogamy and assembly of the MAC genome from MIC sequences. The latter process involves developmentally programmed genome rearrangements controlled by non-coding RNAs and a specialized RNA interference machinery. We describe our first attempts to identify genes and biological processes that contribute to the progression of the sexual cycle. Given the high percentage of unknown genes annotated in the P. tetraurelia genome, we applied a global strategy to monitor gene expression profiles during autogamy, a self-fertilization process. We focused this pilot study on the genes carried by the largest somatic chromosome and designed dedicated DNA arrays covering 484 genes from this chromosome (1.2% of all genes annotated in the genome). Transcriptome analysis revealed four major patterns of gene expression, including two successive waves of gene induction. Functional analysis of 15 up-regulated genes revealed four that are essential for vegetative growth, one of which is involved in the maintenance of MAC integrity and another in cell division or membrane trafficking. Two additional genes, encoding a MIC-specific protein and a putative RNA helicase localizing to the old, then to the new MAC, are specifically required during sexual processes. Our work provides a proof of principle that genes essential for meiosis and nuclear reorganization can be uncovered following genome-wide transcriptome analysis.

Item Type:Article
Uncontrolled Keywords:Gene expression pattern, Meiosis, Nuclear reorganization, Ciliates, RNAi
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions:Laboratory of DNA Sequencing and Oligonucleotide Synthesis
ID Code:32
Deposited By: PhD Jacek Nowak
Deposited On:31 Jan 2011 07:09
Last Modified:05 Oct 2015 08:18

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