Table I 
Sequenced genomes of Staphylococcus Twort-like phages

Bacteriophagea
	Genome
size (bp)b 	LTR size
(bp)	%GC	Number 
of ORFsc	Number
of tRNAs	Number
of intronsd	GenBank
Acc. No.	Source or
reference	
A5W	137,087	8455	30.47	214	4	3	EU418428	Ulatowska et al., in preparation; this work 	
Staph1N	137,192	8455	30.46	214 	 4 	3	JX080300	This work	
Fi200W 	140,079	8402	30.44	   216    	4	 5+1*	JX080303	This work	
P4W 	139,173	8417	30.44	 214 	 4   	 5+1*	JX080305	This work	
676Ż	140,115	8447	30.45	 216 	4 	 5+1* 	JX080302	This work	
A3R 	132,712	8306	30.53	 196 	4	  5+1*  	JX080301	This work	
MSA6	140,194	8049	30.32	214	    4     	  5  	JX080304	This work	
G1	138,715	ND (8432)	30.39	   215   	4	5	AY954969	Kwan et al., 2005	
ISP	138,339	ND (8422)	30.42	  217  	4	3	FR852584	Vandersteegen et al., 2011	
K	127,395	ND (5596)	30.60	     184      	4	3	AY176327	O’Flaherty et al., 2004	
Sb-1	127,188	ND (3959)	30.48	  183   	4	3	HQ163896	Kvachadze et al., 2011	
Twort	130,706	ND	30.26	196 	1	 7 	NC_007021	Kwan et al., 2005	

aBacteriophages Staph1N, Fi200W, P4W, 676Ż, and A3R are derived from the therapeutic phage collection of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wrocław, Poland (Międzybrodzki et al., 2012). Phages A3R and Fi200W are descendants of phages A3 and Fi200, which were obtained from Gerhard Pulverer, Institute of Hygiene, University of Cologne, Germany in 1986, and subsequently adapted to therapeutic applications by several rounds of passaging through certain clinical S. aureus strains, to broaden their range of specificity. Phage P4W corresponds to a clone of phage P4 that is a descendant of fi131 - a phage originally isolated as infecting S. epidermidis and obtained from Hans Lodenkemper, Endoklinik, Hamburg, Germany in 1979. Phage 676Ż is a descendant of phage 676 obtained from Stefan Kryński, Department of Microbiology, Medical Academy, Gdańsk, Poland in 1978. P4W and 676Ż were adapted for therapeutic applications similarly as A3R and Fi200W. Phage Staph1N was obtained from a contaminated culture of PS80 strain. It is possibly a descendant of A5W, which acquired multiple point mutations. Phages that served as a source of DNA for sequencing were propagated in cells of the following host strains: PS80 (Staph1N), 6409 (Fi200W, P4W), R19930 (A3R), and Ż11788 (676Ż). The former two strains are standard strains for phage propagation, whereas the latter two strains are clinical S. aureus isolates. Preparation of DNA was as described elsewhere (Ulatowska et al., in preparation). Pyrosequencing reads from Roche 454 Genome Sequencer GS FLX (with up to a 100-fold coverage), obtained at the Laboratory of Sequencing and Oligonucleotide Synthesis of the Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland, served as a source of data for the assembly of crude versions of phage genomic sequences with use of the Lasergene 9.0 program. Sequences of repeats and ambiguous sites were determined additionally by direct sequencing with specific primers using the ABI PRISM dye terminator cycle sequencing ready reaction kit with ApliTaq DNA polymerase, FS (Perkin Elmer), and ABI 377 automated sequencers (Perkin Elmer) for the separation of reaction products and reading the sequencing results. Physical maps of virion DNA to verify the correctness of sequence assembly were prepared using restriction enzymes (AvaI, AvaII, NdeI, NsbI, KpnI, PflMI, PstI, SacI, SmaI, and XhoI) by analysis of single or double digests.  
bSequences of terminal repeat regions of Staph1N, Fi200W, P4W, 676Z˙ , A3R, and MSA6 virion DNA molecules were determined as described elsewhere (Ulatowska et al., in preparation).Terminal 9- to 12-kb restriction fragments of virion DNA molecules, identified by Bal31 nuclease digestion, separated by electrophoresis in 0.7% agarose gel from other fragments of virion DNA, and isolated from the gel, were used as templates for sequencing. Numbers in parentheses indicate the size of DNA regions (in bp) homologous to regions of known LTRs in other phages. ND, not described.
cCalculation of ORF number in the genomes of all phages, except phage Twort, is based on data in Table II. Calculation of ORF number in intron-containing regions was performed by the addition of intron-encoded ORFs and ORFs obtained after in silico splicing of relevant sequences.
dAsterisks indicate an intron-like sequence immediately downstream of protein-coding gene (tmpA).